Human Stem Cell Core

 

Our mission is to provide expertise and quality-control reagents for culture and differentiation of human ESCs and induced pluripotent stem cells (iPSCs) to CHOP, the University of Pennsylvania, and outside academic communities. 

The facility maintains six NIH-approved hESC lines and several iPSC lines.  Stringent protocols are established to:

  • Propagate human ESC and iPSC lines, monitor for maintenance of the pluripotent state and distribute these lines to investigators
  • Differentiate hESCs/iPSCs into mesoderm and their derivative tissues under controlled, serum-free conditions with defined cytokines
  • Generate iPSCs from human subjects using a single lentiviral vector in which the reprogramming genes can be removed by Cre-mediated recombination

In addition to these standard protocols, we will work with investigators to design new protocols tailored for their individualized research. Technologies for creating and manipulating ESCs/iPSCs are evolving rapidly and we are dedicated to incorporating the latest technologies and protocols into our facility infrastructure.

Services

Training Course: hESC/iPSC Growth and Maintenance

Links

Center for Cellular and Molecular Therapeutics

Contact

 

Deborah L. French, PhD
Director, hESC/iPSC Core Facility
CTRB 5014
(267)426-9673
This email address is being protected from spambots. You need JavaScript enabled to view it.

Paul Gadue, PhD
Co-Director, hESC/iPSC Core Facility
CTRB 5012
(267)426-9607
This email address is being protected from spambots. You need JavaScript enabled to view it.

Mitchell Weiss, MD, PhD
Co-Director, hESC/iPSC Core Facility
Abramson Bldg 316B
(215)590-0565
This email address is being protected from spambots. You need JavaScript enabled to view it.




Home Core Facilities Human Stem Cell Core