Human Pluripotent Stem Cell Core

The Human Pluripotent Stem Cell (PSC) Core was established in 2008 as part of the Center for Cellular and Molecular Therapeutics.  Our mission is to provide expertise and quality-control reagents for the culture and differentiation of human PSCs to CHOP, the University of Pennsylvania, and outside academic communities.  An infrastructure and solid foundation has been created for the generation of iPSC lines that are used by investigators worldwide for modeling human disease to study mechanism, development, and establish new therapeutic modalities.  A recent addition to the core has been the establishment of the genome editing technology using CRISPR/Cas. This technology is being utilized on iPSCs established by the Core for creating isogenic lines that eliminate the clonal heterogeneity and variability in downstream applications. We provide enrichment courses to actively train investigators with the necessary tools and expertise to maximize successful outcomes.

We provide services to the global research community using cutting edge technologies.
Stringent protocols have been established to:

  • Generate iPSCs from human cells using non-integrating reprogramming systems
  • Differentiate human PSCs into the three germ layers and their derivative tissues
  • Perform genome editing on PSC lines for gene targeting and/or transgene expression
  • Propagate and distribute human PSC lines to investigators
  • Provide tested, quality-controlled cell culture reagents to investigators

Services

Current Service listed is the training course found at this link:

Training Course: hESC/iPSC Growth and Maintenance

In development

If you are interested in using a core service please contact the core at This email address is being protected from spambots. You need JavaScript enabled to view it..

Embryonic stem cell (ESC) line distribution

The core maintains four NIH-approved ESC lines:

H1 or WA01, 0043
H9 or WA09, 0062
CHB6, 0006
CHB8, 0007

Investigators must go to the NIH stem cell registry website at http://stemcells.nih.gov/research/registry/ and obtain permission from the appropriate institution for the core to distribute the ESC line. This will include a fee and MTA to the respective institution.

Links

Center for Cellular and Molecular Therapeutics

Forms

Internal Account Registration Form
External Account Registration Form

PSC core billing account

  • Form signed by business manager
  • Email to This email address is being protected from spambots. You need JavaScript enabled to view it.to setup account

Service request

  • In development

Contact

Deborah L. French, PhD
Director
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Paul Gadue, PhD
Co-Director
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Jean Ann Maguire, PhD
Technical Director
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